Premium
Cerebellar defect and impaired motor coordination in mice lacking vimentin
Author(s) -
ColucciGuyon Emma,
Giménez Y Ribotta Minerva,
Maurice Tangui,
Babinet Charles,
Privat Alain
Publication year - 1999
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/(sici)1098-1136(19990101)25:1<33::aid-glia4>3.0.co;2-j
Subject(s) - vimentin , cerebellum , intermediate filament , biology , glial fibrillary acidic protein , intermediate filament protein , neuroscience , astrocyte , neuroglia , ultrastructure , motor coordination , nervous system , central nervous system , microbiology and biotechnology , anatomy , cytoskeleton , cell , immunology , immunohistochemistry , genetics
Vimentin belongs to the family of intermediate filament (IF) proteins. During the nervous system development in mammals, it is transiently expressed in precursor cells of neuronal and glial lineages, and then it is progressively replaced by other types of IF proteins. Surprisingly, mice knock‐out for vimentin develop and reproduce without any apparent defects (Colucci‐Guyon et al. Cell 79:679–694, 1994). In adult rodents, Bergmann glia (BG) of the cerebellum continue to express vimentin together with glial fibrillary acidic protein (GFAP). A careful analysis of cerebellar morphology and ultrastructure in mutants showed poorly developed and highly abnormal BG, whereas the migration of granular neurons proceeded normally. Moreover, many Purkinje cells (PC) appeared stunted with a loss of spiny branchlets, and some of them were necrotic. Finally, impaired motor coordination was evidenced by behavioral tests. These observations demonstrate a role for vimentin in contributing to the normal development and morphology of BG and reveal a hitherto unreported functional relationship between BG and PC. GLIA 25:33–43, 1999. © 1999 Wiley‐Liss, Inc.