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Absence of polyomavirus JC in GLIA brain tumors and glioma‐derived cell lines
Author(s) -
Herbarth Beate,
Meissner Hildegard,
Westphal Manfred,
Wegner Michael
Publication year - 1998
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/(sici)1098-1136(199804)22:4<415::aid-glia11>3.0.co;2-z
Subject(s) - papovavirus , oligodendroglioma , biology , jc virus , astrocytoma , glioma , virology , human brain , virus , cell culture , brain tumor , glial tumor , polymerase chain reaction , progressive multifocal leukoencephalopathy , pathology , cancer research , gene , neuroscience , genetics , medicine
The human neurotropic papovavirus JC, a close relative of simian virus 40, has been associated with the formation of brain tumors in humans because of its ability to induce such tumors in other primates under experimental conditions. Here we have analyzed 30 brain tumors classified as either oligodendroglioma or astrocytoma and 22 cell lines derived from human gliomas for the presence of JC viral sequences using polymerase chain reaction with two different sets of primers. None of the tumors or cell lines contained JC viral sequences. Similarly, we failed to detect expression of JC T antigen in any of 26 human glioma lines analyzed in this study. We conclude that JC virus is not a major cause of human brain tumors. GLIA 22:415–420, 1998. © 1998 Wiley‐Liss, Inc.