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Compartmental distribution of sulfated glycosaminoglycans in lateral and medial midbrain astroglial cultures
Author(s) -
GarciaAbreu J.,
Silva Luiz Claudio F.,
Tovar Fernanda F.,
Onofre Glaucia R.,
Cavalcante Leny A.,
Neto Vivaldo Moura
Publication year - 1996
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/(sici)1098-1136(199608)17:4<339::aid-glia8>3.0.co;2-u
Subject(s) - glycosaminoglycan , biology , neuroglia , sulfation , microbiology and biotechnology , midbrain , chondroitin sulfate , heparan sulfate , compartment (ship) , extracellular , intracellular , neurite , astrocyte , laminin , neuroscience , extracellular matrix , central nervous system , biochemistry , oceanography , in vitro , geology
Sulfated glycosaminoglycans (S‐GAGs) were isolated from the pericellular (P), intracellular (I), and extracellular (E) compartments of astrocytes cultures from lateral (L) and medial (M) sectors of embryonic mouse midbrain; these sectors differ in their ability to support neurite growth (L, permissive, M, non‐permissive for growth) and laminin deposition patterns (L, fibrillar; M, punctate pattern). The total amount of S‐GAGs in M cultures was twice that in L cultures and was particularly high in the P compartment of M glia. Both glial cultures showed heparan sulfate (HS) in the three cellular compartments but chondroitin sulfate (CS) GAGs were vestigial in I and P compartments of L glia. Our results suggest that M and L astrocytes are heterogeneous concerning the ability to synthesize GAGs and distribute them among the different cellular compartments. Together with other data (Garcia‐Abreu et al: J Neurosci Res 40:471, 1995; Garcia‐Abreu et al: Neuroreport 6:761, 1995), the present results suggest that this heterogeneous features might be at least partially responsible for the differential effects of L and M glial cultures on the growth of midbrain neurons and may also be involved in complex ways in the guidance of axons at the brain midline. © 1996 Wiley‐Liss, Inc.