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Granulocyte macrophage colony stimulating factor stimulates in vitro proliferation of astrocytes derived from simian mature brains
Author(s) -
Guillemin Gilles,
Boussin François D.,
Le Grand Roger,
Croitoru Juliana,
Coffigny Herve,
Dormont Dominique
Publication year - 1996
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/(sici)1098-1136(199601)16:1<71::aid-glia8>3.0.co;2-e
Subject(s) - biology , astrocyte , microglia , autocrine signalling , neuroglia , macrophage , granulocyte macrophage colony stimulating factor , immunology , microbiology and biotechnology , astrocytosis , colony stimulating factor , cytokine , tumor necrosis factor alpha , in vitro , central nervous system , receptor , inflammation , haematopoiesis , neuroscience , immunohistochemistry , biochemistry , stem cell
In the brain, granulocyte‐macrophage colony stimulating factor (GM‐CSF) may be released by infiltrated cells of the immune system including T and B lymphocytes and mononuclear phagocytes, but also by nervous system resident cells such as microglia and astrocytes. Astrocyte‐secreted GM‐CSF may play an important role in enhancing the local inflammatory response to central nervous system (CNS) injury and in recruting microglia and activated macrophages. In this study, we demonstrated that GM‐CSF, as TNFα and IL 6, stimulates in vitro proliferation of simian astrocytes in primary cultures. Results were confirmed by blocking experiments performed with a specific neutralizing mAb directed against GM‐CSF. Furthermore, we demonstrated that GM‐CSF mediates its effect on these cells through the α subunit of the GM‐CSF receptor which is constitutively expressed at the membrane of the cultured simian astrocytes as assessed by immunofluorescence. GM‐CSF effects on astrocytes could be involved in astrocytosis, a hallmark of various neurological injuries and in inflammatory processes in an autocrine manner. © 1996 Wiley‐Liss, Inc.