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Quality evaluation of plateletpheresis using the new AMICUS (Baxter) cell separator: Evolution of CD 62 expression
Author(s) -
Laurencet France M.,
Doucet Arlette,
Lydiate Valmay,
Jacquier MarieClaude,
Mermillod Bernadette,
Andersen Sylvia,
Chapuis Bernard
Publication year - 1998
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/(sici)1098-1101(1998)13:2<47::aid-jca1>3.0.co;2-8
Subject(s) - medicine , plateletpheresis , separator (oil production) , immunology , apheresis , platelet , physics , thermodynamics
The purpose of this study was to evaluate the new AMICUS (Baxter‐Fenwal Division) cell separator in terms of donor safety, efficiency, and quality of the product obtained. One hundred eighty‐three single‐donor plateletpheresis procedures were performed, using a collection of 4–4.5 × 10 11 platelets as endpoint. During the first part of the study, the mean volume processed was 3,225 ml and the mean procedure duration 69.5 min. During the second part, after a software change, the mean volume and mean procedure time were 3,071 ml and 68.3 min, respectively. According to local policy, every collection bag was separated into two therapeutic units each containing a mean of 1.87 (1.83) × 10 11 platelets. The white blood cell (WBC) contamination per therapeutic unit was less than 5 × 10 6 in 91% of phereses performed in part one of the study and in 98% of phereses performed in part two. During the recommended 5 days storage, sequential in vitro analyses were performed in 27 units, showing limited platelet activation according to CD62 expression and morphological changes on electron microscopy (EM). Furthermore, there was a correlation between CD62 expression and the degree of WBC contamination ( P = 0.03). In conclusion, platelet collection with the new Amicus allows for high platelet yields of adequate quality as judged by WBC content, CD62 expression, and electron microscopic morphological changes. J. Clin. Apheresis 13:47–55, 1998. © 1998 Wiley‐Liss, Inc.