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Ultraviolet‐B irradiation of leukapheresis products: Dose‐response relationship with the mixed lymphocyte reaction
Author(s) -
Foster Preston F.,
Gebel Howard,
McLeod Bruce C.,
Ebert Nancy,
Jensik Stephen,
Sankary Howard N.,
Tambur Anat,
Williams James W.
Publication year - 1996
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/(sici)1098-1101(1996)11:2<55::aid-jca1>3.0.co;2-9
Subject(s) - leukapheresis , mixed lymphocyte reaction , medicine , immunology , lymphocyte , irradiation , andrology , transplantation , blood irradiation therapy , immune system , pathology , stem cell , t cell , biology , genetics , physics , alternative medicine , cd34 , nuclear physics
Ultraviolet‐B (UVB) irradiation of blood constituents intensifies their anti‐rejection effect in pretransplant donor‐specific transfusions. UVB‐induced inhibition of the mixed lymphocyte reaction (MLR) between UVB‐irradiated donor cells and prospective recipient cells is a predicator of this anti‐rejection effect. In order to define the dose‐response relationship between the incident UVB irradiation on leukocyte concentrates and subsequent inhibition of their MLR responses, we collected 4 ± 2 × 10 9 leukocytes (93 ± 7% lymphocytes) in 200 ml plasma from each of three volunteers by leukapheresis and exposed them to rapid, serial doses of UVB irradiation which was delivered by a blood product irradiator (4R4440 UVB Irradiator, Baxter, Inc) with aliquots removed between doses. Lymphocytes from each aliquot were placed in MLR with panel donors and studied in three groups: 1) the panel donor cells were γ‐irradiated (1,500 rads) (i.e., only the UVB‐irradiated cells could proliferate), 2) the UVB‐irradiated cells were γ‐irradiated (i.e., only the panel lymphocytes could proliferate), and 3) no γ‐irradiation (i.e., both cell populations could proliferate). Each group had a similar UVB dose‐related diminution in the MLR ( p = .79, ANOVA). A single dose of 6 J/cm 2 extinguished the MLR to baseline in all groups. This dose should theoretically prevent transfused cells from producing either graft‐versus‐host disease or allosensitization, and might heighten their tolerogenic effect. This dose will be employed in our study of donor‐specific leukocyte transfusion in clinical renal transplantation. © 1996 Wiley‐Liss, Inc.