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High‐avidity anti‐DNA antibody removal from the serum of systemic lupus erythematosus patients by adsorption using dextran sulfate cellulose columns
Author(s) -
Matsuki Yasunori,
Suzuki Kimihiro,
Kawakami Makoto,
Ishizuka Toshiaki,
Kawaguchi Yasushi,
Hidaka Toshihiko,
Nakamura Haruo
Publication year - 1996
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/(sici)1098-1101(1996)11:1<30::aid-jca7>3.0.co;2-c
Subject(s) - medicine , avidity , antibody , dextran , immunology , cellulose , autoantibody , systemic lupus erythematosus , chromatography , disease , biochemistry , chemistry
The purpose of this study is to determine whether immunoadsorption treatment using a dextran sulfate (DS) column can remove high‐avidity anti‐double‐stranded DNA (anti‐dsDNA) antibody from the blood of patients with systemic lupus erythematosus (SLE). Before and after each immunoadsorption therapy routine, titers of the high‐avidity anti‐dsDNA antibody of 11 SLE patients were measured by using a newly developed assay kit to exclusively detect high avidity anti‐DNA antibody. Patients with active SLE showed significantly higher titers of high‐avidity antibody than did those with inactive SLE, and their titers were significantly reduced by immunoadsorption procedures. Removal of high‐avidity antibodies in vitro was also confirmed by mixing patients' sera and DS gel. Immunoadsorption therapy using DS columns is effective in the removal of high‐avidity anti‐dsDNA antibodies that are closely associated with pathogenicity in SLE. © 1996 Wiley‐Liss, Inc.