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Image processing approaches to biological three‐dimensional electron microscopy
Author(s) -
Herman Gabor T.,
Marabini Roberto,
Carazo JoséMaría,
Garduño Edgar,
Lewitt Robert M.,
Matej Samuel
Publication year - 2000
Publication title -
international journal of imaging systems and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 47
eISSN - 1098-1098
pISSN - 0899-9457
DOI - 10.1002/(sici)1098-1098(2000)11:1<12::aid-ima3>3.0.co;2-n
Subject(s) - image processing , computer science , electron microscope , microscopy , biological specimen , image (mathematics) , sample (material) , electron tomography , artificial intelligence , nanotechnology , biological system , materials science , optics , chemistry , biology , physics , scanning transmission electron microscopy , chromatography
Electron microscopy is a powerful technique for imaging complex biological macromolecules in order to further the understanding of their functions. When combined with sufficiently careful sample preparation procedures that preserve the native structure of the macromolecules and with sophisticated image processing procedures, electron microscopy can lead to very informative estimates of the three‐dimensional (3D) structures of the specimens under study. 3D reconstruction from electron microscopic data is achieving high goals and exceeding expectations unthinkable only a few years ago. However, there are still some areas where either not enough work has been invested or the work has not as yet been fruitful. We describe image processing approaches that shed further light on some of these difficult areas. © 2000 John Wiley & Sons, Inc. Int J Imaging Syst Technol, 11, 12–29, 2000