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Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies: Sequence‐specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE)
Author(s) -
Holloway John W.,
Beghé Bianca,
Turner Steve,
Hinks Lesley J.,
Day Ian N.M.,
Howell W. Martin
Publication year - 1999
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/(sici)1098-1004(199910)14:4<340::aid-humu10>3.0.co;2-z
Subject(s) - taqman , biology , typing , single nucleotide polymorphism , computational biology , oligonucleotide , genomic dna , snp , genetics , molecular inversion probe , polymerase chain reaction , genotype , dna , gene
In the near future the number of SNPs identified and mapped will increase and the need for high throughput SNP typing will be paramount for comprehensive examination by association of the role of genomic regions in disease traits. A range of higher throughput methods for typing SNPs is now in routine use in many laboratories worldwide. In this report, we analyse the relative advantages and disadvantages of three such methods, TaqMan, PCR‐SSOP, and ARMS‐MADGE, currently in use in our laboratories. Throughputs achievable are similar, but there are major differences in cost and time for set‐up, equipment, consumables, and staff time, which may determine the choice for individual laboratories. Hum Mutat 14:340–347, 1999. © 1999 Wiley‐Liss, Inc.