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Prevalence of Lithuanian mutation among St. Petersburg Jews with familial hypercholesterolemia
Author(s) -
Mandelshtam Mikhail,
Chakir Khalid,
Shevtsov Sergei,
Golubkov Valery,
Skobeleva Natalya,
Lipovetsky Boris,
Konstantinov Vladimir,
Denisenko Alexander,
Gaitskhoki Vladimir,
Schwartz Eugene
Publication year - 1998
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/(sici)1098-1004(1998)12:4<255::aid-humu6>3.0.co;2-e
Subject(s) - mutation , biology , genetics , familial hypercholesterolemia , polymorphism (computer science) , single strand conformation polymorphism , polymerase chain reaction , judaism , hum , population , st petersburg , microbiology and biotechnology , gene , demography , genotype , medicine , theology , history , endocrinology , cholesterol , pathology , metropolitan area , sociology , philosophy , performance art , art history
We used polymerase chain reaction‐single‐strand conformation polymorphism (PCR‐SSCP) analysis to detect LDL receptor gene defects in the St. Petersburg population. We have found a deltaG197 mutation in several patients of Jewish origin. The mutation named is shown to be responsible for one‐third (7/23) of familial hypercholesterolemia (FH) cases in St. Petersburg Jews and absent in patients of Russian descent. The prevalence of a deltaG197 mutation in St. Petersburg Jews is consistent with its origin in Lithuania or Poland. The deltaG197 mutation can be easily detected in polyacrylamide minigels because of formation of specific heteroduplexes during PCR with DNA of heterozygous patients. Taken together with high prevalence of the mutation in St. Petersburg Jews, this observation provides an opportunity for DNA diagnostics of FH in this ethnic group. Hum Mutat 12:255–258, 1998. © 1998 Wiley‐Liss, Inc.