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A frequent mutation in the acidic proline‐rich protein gene, PRH2, causing a Q147K change closely adjacent to the bacterial binding domain of the cognate salivary PRP (Pr1′) in Afro‐Americans
Author(s) -
Azen Edwin A.
Publication year - 1998
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/(sici)1098-1004(1998)12:1<72::aid-humu18>3.0.co;2-w
Subject(s) - biology , gene , genetics , transversion , amino acid , saliva , mutation , population , microbiology and biotechnology , biochemistry , demography , sociology
The human salivary proline‐rich proteins (PRPs) are coded by six closely linked genes on chromosome 12p13.2. Two of the PRP genes, PRH1 and PRH2, code for acidic PRPs that are functionally important in binding to teeth surfaces, in maintaining a supersaturated concentration of salivary calcium, and in promoting adherence of potentially pathogenic oral bacteria to the teeth surfaces. A frequent acidic PRP electrophoretic variant is found in Afro‐Americans and was presumed to be located in the 44 amino acid carboxy‐terminal portion of the full length protein (150 amino acids). This region (and especially the carboxy‐terminal dipeptide) may be crucial in promoting bacterial adherence to teeth surfaces. We here report, by DNA sequence analysis, a C→A(2957) transversion that causes a Q147K change in the PR1′ protein. The mutation is located two residues from the carboxy‐terminal dipeptide and thus might influence its bacterial adherence property. We also report a PCR‐based assay for the C→A(2957) mutation and show its applicability in an Afro‐American population (n=61) where the allele frequency (as determined from phenotyping of saliva proteins and PCR DNA analysis) was found to be notably high (0.16). Hum Mutat 12:72, 1998. © 1998 Wiley‐Liss, Inc.