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Mutation analysis of interleukin‐5 in an asthmatic cohort
Author(s) -
Pereira Emma,
Goldblatt Jack,
Rye Peter,
Sanderson Colin,
Le Souëf Peter
Publication year - 1998
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/(sici)1098-1004(1998)11:1<51::aid-humu8>3.0.co;2-o
Subject(s) - biology , cohort , mutation , interleukin 2 , immunology , genetics , medicine , cytokine , gene
Interleukin‐5 (IL‐5) is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. Mutation detection studies were performed on the IL‐5 gene and the α‐chain of its receptor in 30 asthmatic and 30 nonasthmatic subjects. Single‐strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) did not reveal any change from the reported normal sequence in all 4 exons of IL‐5 as well as the promoter and 3′‐untranslated regions of the gene. No SSCP variations were seen within the complete coding sequence of the IL‐5 receptor α‐chain. Mutations of the IL‐5 gene coding region, its promoter and receptor are unlikely to be common causes of an inherited predisposition to asthma. Hum Mutat 11:51–54, 1998. © 1998 Wiley‐Liss, Inc.