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An efficient and reliable multiplex PCR‐SSCP mutation analysis test applied to the human E‐cadherin gene
Author(s) -
Berx G,
Nollet F,
Strumane K,
van Roy F
Publication year - 1997
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/(sici)1098-1004(1997)9:6<567::aid-humu11>3.0.co;2-0
Subject(s) - biology , genetics , multiplex , multiplex polymerase chain reaction , gene , mutation , microbiology and biotechnology , computational biology , polymerase chain reaction
The invasion suppressor gene E‐CADHERIN (CDH1) is downregulated in a large variety of human carcinomas. Up to now, mutational analysis of the CDH1 gene has been described for 325 tumors derived from only four different tissue types. A simple but sensitive mutation detection assay is needed to screen many more tumor types, possibility bearing E‐cadherin inactivating mutations. For that purpose, we developed a multiplex PCR‐SSCP analysis for all 16 CDH1 exons. Ease of experimentation was combined with reliable sensitivity. Indeed, the present multiplex analysis reduces the number of manipulations to 50%, while the mutation detection turned out to be highly efficient and sensitive. Hum Mutat 9:567–574, 1997. © 1997 Wiley‐Liss, Inc.