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Sensitive and fast mutation detection by solid phase chemical cleavage
Author(s) -
Hansen Lise Lotte,
Justesen Just,
Kruse Torben A.
Publication year - 1996
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/(sici)1098-1004(1996)7:3<256::aid-humu10>3.0.co;2-1
Subject(s) - cleavage (geology) , biology , dna , mutation , dna sequencing , microbiology and biotechnology , genetics , computational biology , gene , paleontology , fracture (geology)
We have developed a solid phase chemical cleavage method (SpCCM) for screening large DNA fragments for mutations. All reactions can be carried out in microtiterwells from the first amplification of the patient (or test) DNA through the search for mutations. The reaction time is significantly reduced compared to the conventional chemical cleavage method (CCM), and even by using a uniformly labelled probe, the exact position and nature of the mutation can be revealed. The SpCCM is suitable for automatization using a workstation to carry out the reactions and a fluorescent detection‐based DNA sequencing system to analyze the cleaved fragments. © 1996 Wiley‐Liss, Inc.