Open AccessImpact of anaerobic growth conditions on toxic shock syndrome toxin‐I production by Staphylococcus aureus
Author(s) -
Lungren Max,
Chaisilwattana Pongsakdi,
Knoop Floyd C.,
Monif Gilles R. G.
Publication year - 1996
Publication title -
infectious diseases in obstetrics and gynecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.656
H-Index - 48
eISSN - 1098-0997
pISSN - 1064-7449
DOI - 10.1002/(sici)1098-0997(1996)4:4<232::aid-idog6>3.0.co;2-0
Subject(s) - anaerobic exercise , staphylococcus aureus , toxin , toxic shock syndrome , microbiology and biotechnology , biology , titer , food science , chemistry , bacteria , immunology , physiology , genetics , antibody
Objective The impact of anaerobic growth conditions on the Staphylococcus aureus toxic shock syndrome toxin‐1 (TSST‐1) production was studied. Methods Ten strains of S. aureus derived from patients with toxic shock syndrome (TSS), 10 isolates of S. aureus, and documented TSST‐1‐producing strains recovered from patients with either staphylococcal septicemia or staphylococcal nongenital abscesses were grown under aerobic and anaerobic conditions. The bacterial growth was measured using optical density (OD) determinations at 520 nm. The toxin production was assayed using the TS‐RPLA latex agglutination test. Results Both TSS and non‐TSS strains of S. aureus grown under aerobic and anaerobic conditions exhibited comparable OD patterns of growth, and the levels of toxin production remained constant during the logarithmic phase. Toxin titers developed during the logarithmic growth phase and peaked after 24 h of incubation. When stationary‐phase isolates grown initially under aerobic conditions were subjected to strict anaerobic conditions, subsequent toxin titers, compared with isolates grown in the continued presence of oxygen, were depressed 2‐fold, peaking at a later time. Conclusions TSST‐1 production is diminished under continued anaerobic conditions. © 1997 Wiley‐Liss, Inc.