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Simultaneous saccharification and fermentation of pretreated Antigonum leptopus (Linn) leaves to ethanol
Author(s) -
Krishna S Hari,
Chowdary G V,
Reddy D Srirami,
Ayyanna C
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199911)74:11<1055::aid-jctb148>3.0.co;2-z
Subject(s) - cellulase , trichoderma reesei , hydrolysis , fermentation , chemistry , substrate (aquarium) , ethanol , hydrogen peroxide , food science , ethanol fuel , biochemistry , biology , ecology
Simultaneous saccharification and fermentation (SSF) of alkaline hydrogen peroxide pretreated Antigonum leptopus (Linn) leaves to ethanol was optimized using cellulase from Trichoderma reesei QM‐9414 (Celluclast® from Novo) and Saccharomyces cerevisiae NRRL‐Y‐132 cells. Contrary to the saccharification optima (2.5% w/v substrate concentration, 50 °C, 4.5 pH, 40 FPU cellulase g −1 substrate and 24 h reaction time), the SSF optima was found to be somewhat different (10% w/v substrate, 40 °C, 100 FPU cellulase g −1 substrate and 72 h). Better ethanol yields were obtained with SSF compared with the traditional saccharification and subsequent fermentation (S&F) and when the cellulase was supplemented with β‐glucosidase. © 1999 Society of Chemical Industry