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Quick analysis of enantioselectivity by a chromatographic reactor: II. Experimental verification
Author(s) -
Wu JauYann,
Liu ShiWenn
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199910)74:10<974::aid-jctb136>3.0.co;2-m
Subject(s) - enantioselective synthesis , enantiomer , biocatalysis , naproxen , lipase , chemistry , resolution (logic) , enantiomeric excess , chromatography , chiral stationary phase , candida antarctica , organic chemistry , catalysis , combinatorial chemistry , computer science , enzyme , reaction mechanism , medicine , alternative medicine , pathology , artificial intelligence
A modified chromatographic process was used to determine the enantiomeric ratio ( E ) in enantioselective biocatalysis. Theoretical results indicated that the established relationship, E = ln(1− x S )/ln(1− x R ), was valid for this modified operation. In addition, the Candida cylindracea lipase‐catalyzed enantioselective esterification of racemic naproxen was selected to verify experimentally the effectiveness of the proposed method. The ease of operation and simplicity of data analysis of the proposed method makes it a promising alternative for determining the enantioselectivity of enzymatic chiral resolution. © 1999 Society of Chemical Industry