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Recovery of proteins and other biological compounds using fibrous materials: II. Flocculation by polyelectrolyte addition
Author(s) -
Chen Li Ang,
Carbonell Ruben G,
Serad George A
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199908)74:8<740::aid-jctb112>3.0.co;2-1
Subject(s) - polyelectrolyte , flocculation , carboxymethyl cellulose , biomolecule , cellulose triacetate , adsorption , chemical engineering , bovine serum albumin , chemistry , cellulose acetate , cellulose , polymer , filtration (mathematics) , colloid , materials science , chromatography , polymer chemistry , organic chemistry , biochemistry , statistics , mathematics , engineering , sodium
Polyelectrolytes have been used in wastewater treatment processes to destabilize colloidal suspensions of proteins, cells and other biological compounds, resulting in flocculation. When a solution containing a single model protein, bovine serum albumin (BSA), is treated with a polyelectrolyte, carboxymethyl cellulose (CMC), large and strong flocs are formed, which are easily retained by a 20 µm pore size filter. However, when a mixture of proteins, cells, and fats from an actual wastewater sample is treated in the same manner, smaller and weaker flocs are observed. An adsorption and filtration process for the recovery of valuable biological compounds using cellulose‐based fibrous materials has been developed. When used simultaneously with CMC, cellulose acetate and triacetate fibrets (CAF and CTF) resulted in high recovery of biomolecules from solution at very low dosages of both polyelectrolyte and fibrets. CMC interacts with biomolecules by electrostatic interactions and polymer bridging, while CTF/CAF facilitate floc growth by adsorption and bridging of primary particles and by entrapment of small aggregates within their highly fibrillated microstructure. © 1999 Society of Chemical Industry