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Comparison of prednisolone production from cortexolone by free and immobilized isolated and standard collection strains using mixed culture techniques
Author(s) -
Manosroi Jiradej,
Manosroi Aranya,
Saraphanchotiwittaya Aurasorn,
Abe Masahiko
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199904)74:4<364::aid-jctb37>3.0.co;2-5
Subject(s) - bioconversion , yield (engineering) , bacillus sphaericus , chemistry , bacteria , microbiology and biotechnology , chromatography , nuclear chemistry , biology , organic chemistry , bacillales , fermentation , materials science , bacillus subtilis , metallurgy , genetics
The bioconversion of cortexolone (Reichstein's CompoundS) to hydrocortisone or hydrocortisone to prednisolone byreference and isolated fungi and bacteria were investigated. Thereference strains Cunninghamella echinulata ATCC 8688a and Bacillus sphaericus ATCC 13805, and the isolates Cechinulata SRP III and B sphaericus SRP III were used asfree or immobilized forms in single or mixed cultures by eithersequential or concomitant mixing. In single cultures, immobilizedfungi C echinulata ATCC 8688a and C echinulata SRP IIIshowed less hydrocortisone yield than the free form, whereasimmobilized B sphaericus ATCC 13805 and B sphaericus SRP III gave higher prednisolone yield than the free form. In mixedcultures, free fungi with immobilized bacteria of both isolated andstandard strains showed the highest prednisolone yield both byconcomitant and sequential mixing. The highest prednisolone yield of72.5±0.78% with a bioconversion rate of 0.76% h −1 by sequential mixing was obtained with free Cechinulata ATCC 8688a with immobilized B sphaericus ATCC13805 at 96 h, whereas the free C echinulata SRP III withimmobilized B sphaericus ATCC 13805 and the free Cechinulata SRP III with immobilized B sphaericus SRP IIIgave 63.4±0.74% and 47.7±0.44% at 120 hwith bioconversion rates of 0.53 and 0.40% h −1 respectively. Free C echinulata SRP III with immobilized B sphaericus SRP III gave a maximum yield of52.8±0.82% with a bioconversion rate of 0.44% h −1 at 120 h which was comparable to59.2±0.44% with a bioconversion rate of 0.49% h −1 obtained by free C echinulata ATCC 8688awith immobilized B sphaericus ATCC 13805 using concomitantmixing. This study indicated high biotransformation activity of freefungal and immobilized bacterial isolates which showed comparablesteroidal productivity to the standard strains both in single andmixed cultures by the concomitant mixing technique. © 1999 Society of Chemical Industry