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Processing of nanoparticulate bioproducts: application and optimisation of aqueous two‐phase systems
Author(s) -
Walker Simon G,
Lyddiatt Andrew
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199903)74:3<250::aid-jctb25>3.0.co;2-w
Subject(s) - flocculation , chemistry , fractionation , aqueous solution , chromatography , peg ratio , phosphate , isoelectric point , partition coefficient , nanoparticle , aqueous two phase system , chemical engineering , materials science , nanotechnology , organic chemistry , finance , engineering , economics , enzyme
A simple aqueous two‐phase system (ATPS) has been identified and implemented for the fractionation of small inclusion bodies (IBs) from debris in microbial cell homogenates. Many problems imposed by processing fine particulates are circumvented. At pH 9.4, the greatest IB recoveries were obtained with PEG 8000/phosphate systems (10/10% w/w) where IBs accumulated as a discrete sediment within the salt‐rich lower phase. The majority of micronised debris partitioned as a discrete interphase. Manipulation of the system pH recommended operation above the isoelectric point of the target product to avoid debris flocculation which undermined partition selectivity. System optimisation was achieved by the addition of 0.4 mol dm −3 NaCl (at pH 9.4) where IB recovery increased to 87% of the load concomitant with preferential fractionation of debris to the interphase. The generic application of ATPS to the bioprocessing of nanoparticles is discussed. © 1999 Society of Chemical Industry