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Colloidal gas aphrons generated from the anionic surfactant AOT for the separation of proteins from aqueous solution
Author(s) -
Noble Matthew J,
Varley Julie
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199903)74:3<231::aid-jctb20>3.0.co;2-9
Subject(s) - pulmonary surfactant , chemistry , aqueous solution , colloid , chromatography , downstream processing , chymotrypsinogen , chemical engineering , inner core , biochemistry , organic chemistry , materials science , chymotrypsin , enzyme , trypsin , composite material , engineering
Colloidal gas aphrons are microbubbles 10–100 μm in diameter created by intense stirring of a surfactant solution. They are composed of a gaseous inner core surrounded by a surfactant film. They have a high surface area, are relatively stable and have the ability to interact with proteins through electrostatic interactions. These characteristics make colloidal gas aphrons ideally suited for use as a downstream processing technique. In this study the effect of different process parameters on the recovery of three proteins (conalbumin, ribonuclease A and α‐chymotrypsinogen A) was studied for a protein–AOT (sodium bis‐(2 ethylhexyl) sulfosuccinate) system. High enrichment ratios (up to 8) and high recovery of the protein (85%) are found under optimum conditions. © 1999 Society of Chemical Industry