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Azeotropic phenomena during separation of dilute mixtures of proteins by simulated moving bed chromatography
Author(s) -
Houwing Joukje,
Billiet Hugo AH,
Wesselingh Hans A,
van der Wielen Luuk AM
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199903)74:3<213::aid-jctb46>3.0.co;2-m
Subject(s) - simulated moving bed , chromatography , separation (statistics) , ion chromatography , chemistry , salt (chemistry) , reversing , flow (mathematics) , ion exchange , selectivity , breakthrough curve , ion , mechanics , materials science , computer science , catalysis , organic chemistry , physics , adsorption , machine learning , composite material
Recently, we have introduced the use of a salt gradient over a simulated moving bed system used for purification of proteins using ion exchange chromatography. This paper describes azeotropic phenomena in such a system, where complete separation of mixtures of proteins is not possible at certain salt concentrations, due to a reversing selectivity. These phenomena impose additional restrictions on the selection of the flow rates in each of the sections of the simulated moving bed which are discussed. © 1999 Society of Chemical Industry