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Influence of growth medium composition on the lipolytic enzyme activity of Ophiostoma piliferum (Cartapip TM )
Author(s) -
George Ellen,
Tamerler Candan,
Martinez Angel,
Martinez Maria Jesus,
Keshavarz Tajalli
Publication year - 1999
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199902)74:2<137::aid-jctb997>3.0.co;2-h
Subject(s) - lipase , esterase , chemistry , glycerol , enzyme , composition (language) , food science , biochemistry , growth medium , yeast , carbon source , extracellular , biology , philosophy , linguistics , bacteria , genetics
Abstract Production of esterases (carboxyl esterase EC 3.1.1.1) and lipases (glycerol ester hydrolase EC 3.1.1.3) by Ophiostoma piliferum (Cartapip TM ), a fungus commercialized to decrease fatty acyl esters in wood, is described. The influence of various combinations of carbon and nitrogen sources, in the growth medium, was examined. Medium containing yeast extract as a nitrogen source and olive oil as a carbon source was found to be optimal for extracellular esterase (221 U dm −3 ) and lipase (152 U dm −3 ) activities. Further increases in those enzyme activities were achieved by decreasing medium pH from 6.5 to 5.5 (esterase 508 U dm −3 ; lipase 415 U dm −3 ) and increasing medium calcium content from 8 m mol dm −3 to 160 m mol dm −3 (esterase 4084 U dm −3 ; lipase 508 U dm −3 ) © 1999 Society of Chemical Industry