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A piezoelectric immunosensor for complement C4 using protein A oriented immobilization of antibody
Author(s) -
Pei RenJun,
Hu JiMing,
Hu Yi,
Zeng Yun'e
Publication year - 1998
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199809)73:1<59::aid-jctb917>3.0.co;2-3
Subject(s) - glutaraldehyde , electrode , piezoelectricity , materials science , biosensor , protein adsorption , reproducibility , crystal (programming language) , antibody , adsorption , membrane , chromatography , protein crystallization , adhesion , complement system , nanotechnology , chemistry , biochemistry , composite material , immunology , biology , organic chemistry , programming language , computer science , crystallization
A highly sensitive piezoelectric crystal immunosensor has been developed for the detection of human complement C4. Three methods for immobilization of anti‐C4 antibody on the gold electrodes were employed. The immobilization method with protein A method gave the best results in terms of sensitivity, stability and reproducibility. The coated crystal with either polyethyleneimine adhesion, followed by a glutaraldehyde cross‐linking method, or physical adsorption method, gave inferior results. A thin protein A membrane was formed on the gold electrodes so that C4 antibody could be effectively self‐assembled, as an orderly, closely arranged and oriented antibody membrane on the protein A layer. Under the optimized experimental conditions, the coated crystal had good response to C4 in the range 5×10 −4 to 1×10 −2 g dm −3 . Other proteins in human serum did not interfere with the detection. The crystal could be regenerated nearly 10 times. © 1998 Society of Chemical Industry