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Review: Purification of proteins by membrane chromatography
Author(s) -
Charcosset Catherine
Publication year - 1998
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199802)71:2<95::aid-jctb823>3.0.co;2-j
Subject(s) - membrane , chromatography , chemistry , affinity chromatography , ion chromatography , hydrophilic interaction chromatography , adsorption , membrane protein , ion exchange , high performance liquid chromatography , ion , biochemistry , organic chemistry , enzyme
Adsorption chromatography is increasingly used for protein purification and medical applications. Synthetic membranes have advantages as support matrices in comparison to conventional bead supports because they are not compressible and they eliminate diffusion limitations. As a result, higher throughput and faster processing times are possible in membrane systems. In this paper, we review the various applications of membrane chromatography by focusing on affinity, ion exchange, hydrophobic interaction, reversed‐phase and multistage chromatography. The prospects of further development of membrane chromatography are discussed. © 1998 SCI.