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An isotherm model describing concave‐down scatchard curve for heterogeneous affinity adsorption
Author(s) -
Suen ShingYi
Publication year - 1997
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199711)70:3<278::aid-jctb750>3.0.co;2-8
Subject(s) - adsorption , thermodynamics , chemistry , kinetic energy , membrane , langmuir adsorption model , scatchard plot , sorption isotherm , pepstatin , kinetics , curve fitting , chromatography , mathematics , biochemistry , binding site , physics , enzyme , statistics , quantum mechanics , protease
A new isotherm model, derived for a concave‐down Scatchard curve, is presented to fit the experimental results for pepsin and chymosin adsorption to pepstatin A affinity membranes. The new model is converted into a kinetic expression and used for the fitting of association curves. The results from the new kinetic model show a better agreement with the experimental data than the Langmuir kinetic model. The new kinetic model is also used together with the affinity membrane model to predict the single‐solute breakthrough curves, which performs well in matching the experimental curves. ©1997 SCI