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Enzymatic Hydrolysis of Sunflower Oil: Characterisation of Interface
Author(s) -
Albasi Claire,
Riba J. Pierre,
Sokolovska Ivana,
Bales Vladimir
Publication year - 1997
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199707)69:3<329::aid-jctb712>3.0.co;2-x
Subject(s) - sunflower oil , hydrolysis , lipase , chemistry , steric effects , enzymatic hydrolysis , saturation (graph theory) , chromatography , sunflower , kinetics , enzyme , chemical engineering , organic chemistry , analytical chemistry (journal) , biochemistry , physics , mathematics , combinatorics , quantum mechanics , engineering
The results of a study of the enzymatic hydrolysis of sunflower oil are presented. The influence of the enzyme and substrate concentrations has been investigated. The interfacial area was modified by changing the volume fraction of oil, while keeping other variables constant. A phase inversion is indicated, which influenced the reaction kinetics by the possible formation of enzyme multilayers, because of steric hindrance. By means of the initial hydrolysis rate, the lipase concentration for achieving interface saturation was estimated. A Lewis cell type contactor was used for the determination of the interface surface, and droplet diameters were deduced. © 1997 SCI.