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Whole Cell Biotransformation of 5‐(4‐(2‐(2‐Pyridyl)methylamino)ethoxy)benzylidenethiazolidine‐2,4‐dione to its Benzyl Derivative Using a Yeast Reductase
Author(s) -
Heath Catherine M.,
Imrie Robert C.,
Jones Joanna J.,
Rees Michael J.,
Robins Keith G.,
Verrall Michael S.
Publication year - 1997
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199703)68:3<324::aid-jctb655>3.0.co;2-4
Subject(s) - biotransformation , yeast , chemistry , fermentation , solvent , chromatography , extraction (chemistry) , solubility , derivative (finance) , organic chemistry , biochemistry , enzyme , financial economics , economics
Whole yeast cell reductive biotransformation of a benzylidene thiazolidinedione to its corresponding benzyl derivative has been scaled up from laboratory to small pilot plant scale. The fermentation conditions for the yeast Rhodotorula rubra were examined and cell titres increased by optimising the medium. Problems encountered in handling substrates of low water solubility are discussed. Various techniques are described for adapting the procedure for process scale operation using the general principles of process scale‐up and integration. Liquid–liquid extraction using a dense solvent was performed on whole broth systems using a continuous flow liquid–liquid separator. Fatty acid impurities were removed using selective solvent extraction. © 1997 SCI.