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Response Surface Optimization of the Critical Media Components for the Production of Surfactin
Author(s) -
Sen Ramkrishna
Publication year - 1997
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199703)68:3<263::aid-jctb631>3.0.co;2-8
Subject(s) - surfactin , central composite design , critical micelle concentration , chemistry , pulmonary surfactant , response surface methodology , yield (engineering) , fractional factorial design , factorial experiment , micelle , chromatography , materials science , mathematics , biochemistry , aqueous solution , organic chemistry , metallurgy , genetics , bacillus subtilis , bacteria , biology , statistics
Optimization of the fermentation media for maximization of surfactin production was carried out. The carbon source (glucose), the nitrogen source (ammonium nitrate) and the mineral salts ferrous and manganous sulphates were the critical components of the medium optimized. A 2 4 full factorial central composite experimental design followed by multi‐stage Monte‐Carlo optimization was used in the design of experiments and in the analysis of results. This procedure limited the number of actual experiments performed while allowing for possible interactions between the four components. The optimum values for the tested variables for the maximal production of surfactin were (in g dm −3 ): glucose = 36·5; NH 4 NO 3 = 4·5; FeSO 4 = 4×10 −3 and MnSO 4 = 27·5 ×10 −2 . Relative surfactant concentrations were expressed as the reciprocal of the critical micelle concentration (CMC −1 ) and the maximum predicted yield of surfactin in terms of CMC −1 was 45·5. © 1997 SCI.