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Enzymatic Synthesis of Thioesters in Non‐conventional Solvents
Author(s) -
Caussette Mylène,
Marty Alain,
Combes Didier
Publication year - 1997
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199703)68:3<257::aid-jctb619>3.0.co;2-m
Subject(s) - chemistry , oleic acid , solvent , substrate (aquarium) , chromatography , lipase , yield (engineering) , hexane , supercritical fluid , organic chemistry , enzyme , supercritical carbon dioxide , nuclear chemistry , biochemistry , oceanography , materials science , metallurgy , geology
The feasibility of enzymatic thioesterification between oleic acid and butanethiol in n ‐hexane, with the immobilised lipase (Lipozyme) from Mucor miehei , has been demonstrated. The immobilised enzyme quantity (100 mg), temperature (40°C), pH range (6–9) and water content (10%) were studied and their optimum values were determined. A preliminary kinetic study indicated a low butanethiol affinity for the enzyme ( K m = 1·85 mol dm −3 ). Even when butanethiol was used without solvent, no substrate inhibition was observed. The possibility of carrying out this reaction in a natural solvent, supercritical carbon dioxide (SCCO 2 ), was successfully verified. After 8 h reaction, a conversion yield of oleic acid of about 33% was obtained. © 1997 SCI.