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Application of Immobilized α‐Chymotrypsin to Peptide Synthesis in Frozen Aqueous Systems
Author(s) -
Haensler Marion,
Gerisch Sabine,
Rettelbusch Janett,
Jakubke HansDieter
Publication year - 1997
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199702)68:2<202::aid-jctb605>3.0.co;2-m
Subject(s) - chymotrypsin , peptide , chemistry , aqueous solution , combinatorial chemistry , chromatography , biochemistry , trypsin , enzyme , organic chemistry
In order to combine the high potential of frozen state peptide synthesis and the advantages of the application of immobilized proteases, the capability of carrier‐bound α‐chymotrypsin (CT, EC 3.4.21.1) to form peptide bonds in frozen aqueous reaction mixtures was investigated. The properties of the support materials strongly influenced the peptide yields. CT bound to rather hydrophobic supports catalysed peptide synthesis as effectively as the soluble enzyme. The immobilized enzyme preparations could be successfully re‐used at least 15 times. Peptide synthesis catalysed by immobilized chymotrypsin was also studied in low water organic reaction mixtures at sub‐zero temperatures. The advantages and limitations of the different approaches are discussed. © 1997 SCI.