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A Simple Method for Obtaining Kinetic Equations to Describe the Enzymatic Hydrolysis of Biopolymers
Author(s) -
GonzálezTello P.,
Camacho F.,
Jurado E.,
Guadix E. M.
Publication year - 1996
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199611)67:3<286::aid-jctb566>3.0.co;2-u
Subject(s) - hydrolysis , starch , kinetic energy , simple (philosophy) , function (biology) , thermodynamics , chemistry , rate equation , enzymatic hydrolysis , enzyme , chromatography , mathematical analysis , analytical chemistry (journal) , physics , kinetics , mathematics , organic chemistry , classical mechanics , biology , philosophy , epistemology , evolutionary biology
A procedure to obtain the overall rate of hydrolysis of biopolymers is proposed, based on the fitting of the experimental data x =f( t ) to cubic spline functions and from these, by differentiation, to obtain d x /d t . The values of these d x /d t slopes are an exclusive function of the conversion, x , when E 0 , S 0 , pH and temperature are constant. The fitting of d x /d t versus x leads to equations of the type d x d t = a · x exp(‐ b · x ) for the glucoamylase–starch system, where b =8·75 and a =f( E 0 , T).