Premium
Characterization of composite membrane of poly(vinyl alcohol) and regenerated silk fibroin for immobilization of horseradish peroxidase and an amperometric neckelocene‐mediated sensor sensitive to hydrogen peroxide
Author(s) -
Liu Haiying,
Zhang Xiaolin,
Zhang Zhanen,
Qi Deyao,
Fan Yubo,
Liu Yongcheng,
Liu Shenghui,
Yu Tongyin,
Deng Jiaqi
Publication year - 1996
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199609)67:1<77::aid-jctb526>3.0.co;2-4
Subject(s) - fibroin , horseradish peroxidase , vinyl alcohol , amperometry , hydrogen peroxide , membrane , chemistry , chemical engineering , composite number , scanning electron microscope , polymer chemistry , materials science , silk , electrode , polymer , electrochemistry , organic chemistry , composite material , biochemistry , enzyme , engineering
Horseradish peroxidase (HRP) was effectively entrapped in a novel composite membrane of poly(vinyl alcohol) and regenerated silk fibroin. IR spectroscopy was employed to characterize the structure of the composite membrane and scanning electron microscopy was used to visualize the morphology of the composite membrane. HRP was utilized to amplify the amperometric response of the sensor by catalyzing reduction of hydrogen peroxide via nickelocene, an electron transfer mediator between immobilized HRP and a glassy carbon electrode. The influence of various experimental parameters such as pH, temperature and applied potential were explored for optimum analytical performance. The sensor possessed a variety of characteristics including good sensitivity, rapid response time and a low detection of limit of 0·1 μmol dm −3 , which arose from the efficiency of electron transfer between immobilized HRP and the electrode via nickelocene.