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Large‐scale production of a hypoallergenic preparation of F(ab′) 2 fragments from bovine colostrum
Author(s) -
LefrancMillot Catherine,
VercaigneMarko Dominique,
Dhulster Pascal,
Fertin Bertrand,
Quinque Bernard,
Guillochon Didier
Publication year - 1996
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199605)66:1<79::aid-jctb462>3.0.co;2-k
Subject(s) - colostrum , rennet , chemistry , digestion (alchemy) , chromatography , ultrafiltration (renal) , milking , yield (engineering) , antibody , pepsin , food science , casein , enzyme , biochemistry , zoology , biology , materials science , metallurgy , immunology
Abstract A method for the production of bovine colostral F(ab′) 2 fragments at pilot‐plant scale was developed. Optimum yield of immunoglobulins in colostral whey was obtained after rennet treatment of first milking colostrum. Pepsin digestion was carried out directly on the colostral whey at pH 3·8 for 4 h, which led to the complete digestion of immunoglobulins into F(ab′) 2 fragments. Elimination of β‐lactoglobulin, the main immunogenic protein, was achieved by anion exchange on Duolite A560 at pH 6·0. The preparation was diafiltered with a 5000 Da membrane and the retentate spray‐dried. The powder obtained contained approximately 34% F(ab′) 2 fragments, with an antibody activity three times higher than the initial colostrum.