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Induction of xylose reductase and xylitol dehydrogenase activities on mixed sugars in Candida guilliermondii
Author(s) -
Lee Hung,
Sopher Coralie R.,
Yau Kerrm Y. F.
Publication year - 1996
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199604)65:4<375::aid-jctb437>3.0.co;2-t
Subject(s) - xylose , xylitol , cellobiose , biochemistry , arabinose , catabolite repression , chemistry , fructose , aldose reductase , dehydrogenase , enzyme , fermentation , cellulase , mutant , gene
The ability of various sugars to induce xylose reductase (aldose reductase, EC 1.1.1.21) and xylitol dehydrogenase ( D ‐xylulose reductase, EC 1.1.1.9) activities in Candida guilliermondii was studied. D ‐Xylose was found to be the best inducer of activities of both enzymes, followed closely by L ‐arabinose. Very low xylose reductase activity was induced by cellobiose, D ‐mannose, D ‐glucose, D ‐galactose, D ‐fructose or glycerol. With xylitol dehydrogenase, cellobiose and D ‐fructose caused partial induction of enzyme activity, while negligible activity was induced by D ‐mannose, D ‐glucose, D ‐galactose or glycerol. Several sugars were tested for the ability to repress the induction by D ‐xylose of xylose reductase and xylitol dehydrogenase activities in C. guilliermondii . Enzyme activities induced on D ‐xylose served as controls. L ‐Arabinose, cellobiose and D ‐galactose did not repress enzyme induction by D ‐xylose, while D ‐mannose, D ‐glucose and D ‐fructose repressed enzyme induction to varying extents. Results from enzyme induction generally correlated with patterns of mixed sugar utilization, with some anomalies associated with the utilization of D ‐galactose and D ‐fructose in the presence of D ‐xylose. The results show that the utilization of D ‐xylose by C. guilliermondii is subject to regulation by induction and catabolite repression.