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Biocatalytic production of useful esters by two forms of lipase from Candida rugosa
Author(s) -
Linko YuYen,
Yan Wu Xiao
Publication year - 1996
Publication title -
journal of chemical technology and biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.64
H-Index - 117
eISSN - 1097-4660
pISSN - 0268-2575
DOI - 10.1002/(sici)1097-4660(199602)65:2<163::aid-jctb416>3.0.co;2-c
Subject(s) - candida rugosa , lipase , chemistry , yield (engineering) , oleic acid , candida antarctica , rapeseed , triacylglycerol lipase , fraction (chemistry) , butanol , chromatography , organic chemistry , high performance liquid chromatography , ethanol , enzyme , biochemistry , food science , materials science , metallurgy
Two distinct lipase forms were obtained from Candida rugosa lipase by Phenyl Sepharose hydrophobic interaction and DEAE Sepharose ion exchange chromatography, L1 at 45% yield and L2 at 4·7% yield. Both purified lipases were able to catalyse esterification of 1‐butanol and oleic acid and trans‐esterification of 2‐ethyl‐1‐hexanol and rapeseed oil. Lipase L1 gave a 98% yield for esterification over 12 h and a 99% conversion of rapeseed oil for trans‐esterification over 24 h. The minor fraction L2 gave a 97% yield for esterification over 30 h and only a 79% conversion for trans‐esterification over 24 h. The superiority of fraction L1, especially in trans‐esterification, could be clearly shown by reversed phased HPLC analysis. Sodium deoxycholate treatment of the purified main lipase L1 considerably improved the initial rate in both esterification and trans‐esterification.