Premium
Interaction between CD44 and the repeat domain of ankyrin promotes hyaluronic acid‐mediated ovarian tumor cell migration
Author(s) -
Zhu Dan,
Bourguig Lilly Y.W.
Publication year - 2000
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(200005)183:2<182::aid-jcp5>3.0.co;2-o
Subject(s) - ankyrin , cd44 , ankyrin repeat , transfection , microbiology and biotechnology , hyaluronic acid , biology , cell adhesion , cell adhesion molecule , cell , chemistry , cell culture , biochemistry , genetics , gene
The adhesion molecule, CD44, interacts with ankyrin within its cytoplasmic domain and binds to hyaluronic acid (HA) at its extracellular domain. In this study, we focused on the functional domain in ankyrin (in particular, the ankyrin repeat domain [ARD]) responsible for CD44 binding and its role in regulating HA‐mediated ovarian tumor cell function. Using recombinant fragments of ankyrin (e.g., ARD and subdomain 1 [S1, aa1‐aa217], subdomain 2 [S2, aa218‐aa381], subdomain 3 [S3, aa382‐aa612], and subdomain 4 [S4, aa613‐aa834]) and in vitro binding assays, we determined that the S2 but not S1, S3, or S4 of ARD is the primary ankyrin binding region for CD44. Microinjection of antiglutathione S‐transferase (GST)‐tagged S2 or GST‐tagged ARD fusion protein into CD44‐positive ovarian tumor cells (e.g., SKOV3 cell line) promotes ankyrin association with CD44 in plaque‐like structures and membrane projections. Additionally, we demonstrated that transfection of SKOV3 cells with S2cDNA or ARD cDNA results in an upregulation of HA‐mediated tumor cell migration. Taken together, we believe that the S2 of the ARD plays a pivotal role in the direct binding to CD44 and promotes the cytoskeleton activation required for HA‐mediated function such as ovarian tumor cell migration. J. Cell. Physiol. 183:182–195, 2000. © 2000 Wiley‐Liss, Inc.