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Expression of osteogenic protein‐1 mRNA in cultured kidney cells
Author(s) -
Kitten Allison M.,
Kreisberg Jeffrey I.,
Olson Merle S.
Publication year - 1999
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199912)181:3<410::aid-jcp4>3.0.co;2-h
Subject(s) - microbiology and biotechnology , biology , messenger rna , cell type , cell growth , cell , cell culture , receptor , kidney , medicine , endocrinology , gene , biochemistry , genetics
Osteogenic protein‐1 (OP‐1 or BMP‐7) is a multifunctional cytokine that regulates the development of several tissues during embryogenesis, including the skeleton, eye, and kidney. In postnatal life, OP‐1 expression is most abundant in the kidney, although the cellular localization of this expression has not been described. In this study, we utilized a cell culture approach to localize OP‐1 mRNA expression in various renal cell types and to determine potential target cells for OP‐1 effects. OP‐1 mRNA expression was demonstrated in several glomerular cell types, such as mesangial, epithelial, and endothelial cells. Distal tubule MDCK cells also expressed OP‐1 mRNA but human proximal tubule HK‐2 cells did not. Multiple OP‐1 transcripts, which ranged in size from 1.6 to 3.8 kb, were observed in both glomerular and tubule cells. Interestingly, the pattern of expression varied among the different cell types, suggesting cell‐specific expression of OP‐1 mRNA. Analysis of OP‐1 receptor expression revealed transcripts for BMP receptors type IA and IB in HK‐2 cells and transcripts for BMPR‐IA and ALK‐2 in mesangial cells. Treatment of HK‐2 cells with OP‐1 (300 ng/ml) for 24–48 hr increased cellular proliferation whereas treatment of cells with transforming growth factor‐β had no effect. Mesangial cell proliferation was not affected by OP‐1. The results suggest that OP‐1 is produced in the renal glomerulus and then travels to the proximal tubule to regulate the proliferation of cells in this region of the nephron. J. Cell. Physiol. 181:410–415, 1999. © 1999 Wiley‐Liss, Inc.