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13 C‐NMR investigation of protein synthesis during apoptosis in human leukemic cell lines
Author(s) -
Scott Corey E.,
Adebodun Foluso
Publication year - 1999
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199910)181:1<147::aid-jcp15>3.0.co;2-m
Subject(s) - dexamethasone , apoptosis , protein biosynthesis , cell culture , amino acid , chemistry , cell , microbiology and biotechnology , biology , biochemistry , endocrinology , genetics
In order to evaluate the role of protein synthesis in apoptosis, 13 C‐NMR has been used to study the levels of protein synthesis in three different human leukemic cell lines in the presence and absence of dexamethasone‐induced apoptosis. Measurements were done on one dexamethasone‐sensitive (CEM‐C7‐14) and two different dexamethasone‐resistant variants (CEM‐4R4 and CEM‐ICR27‐4). The incorporation of 13 C‐labeled amino acids into cellular proteins, which reflects the level of new protein synthesis, was monitored by 13 C‐NMR spectroscopy. In the absence of dexamethasone, the level of protein synthesis was found to be significantly different among the three cell lines. Dexamethasone caused a significant reduction (≅60–87%) in the level of protein synthesis in dexamethasone‐sensitive CEM‐C7‐14 cells, while having no significant effect on protein synthesis in dexamethasone‐resistant CEM‐4R4 cells. Dexamethasone treatment caused a significant enhancement of the level of protein synthesis in the CEM‐ICR27‐4 cells. Synthesis of proteins was found to occur during apoptosis, albeit at a low level, suggesting a role for the synthesis of specific proteins in the mechanism of apoptosis. J. Cell. Physiol. 181:147–152, 1999. © 1999 Wiley‐Liss, Inc.