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Retinoic acid effects on an SV‐40 large T antigen immortalized adult rat bone cell line
Author(s) -
LafageProust MarieHelene,
Wesolowski Gregg,
Ernst Matthias,
Rodan Gideon A.,
Rodan Sevgi B.
Publication year - 1999
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199906)179:3<267::aid-jcp4>3.0.co;2-0
Subject(s) - retinoic acid , cell culture , antigen , immortalised cell line , chemistry , line (geometry) , microbiology and biotechnology , biology , immunology , genetics , mathematics , geometry
Clonal cell lines were established from adult rat tibia cells immortalized with SV‐40 large T antigen. One clone (TRAB‐11), in which retinoic acid (RA) induced alkaline phosphatase (AP) activity, was selected for further study. The TRAB‐11 cells express high levels of type I collagen mRNA, type IV collagen, fibronectin, practically no type III collagen, little osteopontin, and no osteocalcin. RA stimulates proliferation of TRAB‐11 cells (starting at 10 pM) and survival (starting at 100 pM). TRAB‐11 cells synthesize fibroblast growth factor‐2 (FGF‐2), which has potent autocrine mitogenic effects on these cells and acts synergistically with RA. TRAB‐11 cells attach better to type IV collagen than to fibronectin or laminin. Cell attachment to type IV collagen is increased by RA and decreased (65%) by an antibody directed against α 1 β 1 integrin. RA up‐regulates steady‐state levels of α 1 mRNA without affecting β 1 mRNA expression. In conclusion, we report the establishment of a clonal cell line from the outgrowth of adult rat tibiae which is highly sensitive to RA in its growth and survival in culture, apparently as a result of integrin‐mediated cell interaction with extracellular matrix proteins. J. Cell. Physiol. 179:267–275, 1999. © 1999 Wiley‐Liss, Inc.