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Peroxisome proliferation, adipocyte determination and differentiation of C3H10T1/2 fibroblast cells induced by humic acid: Induction of PPAR in diverse cells
Author(s) -
Lee Yashang,
Huang TienShang,
Yang MeiLing,
Huang LanRu,
Chen ChingHsein,
Lu FungJou
Publication year - 1999
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199905)179:2<218::aid-jcp12>3.0.co;2-u
Subject(s) - peroxisome , peroxisome proliferator activated receptor , adipocyte , humic acid , flow cytometry , cell growth , fibroblast , chemistry , microbiology and biotechnology , cell , receptor , biochemistry , biology , cell culture , cytotoxicity , peroxisome proliferator activated receptor gamma , adipose tissue , in vitro , fertilizer , genetics , organic chemistry
Humic acid, a high‐molecular‐weight polyphenolic compound, exists abundantly in soil, natural water, and various terrestrial and aquatic environments. Humic acid causes peroxisome proliferation in mouse liver and induces the expression of peroxisome proliferator activated receptor (PPAR) in BNL CL.2 cells. Both cytotoxicity and flow cytometry show that humic acid inhibits the growth of C3H10T1/2 cells at G 1 phase. C3H10T1/2 fibroblast cells express PPARγ and the adipocyte P2 (aP2) genes which convert into adipocytes after being treated with humic acid. Our findings may provide a unique model for studying the molecular control of determination and differentiation of mesodermal cell lineages. J. Cell. Physiol. 179:218–225, 1999. © 1999 Wiley‐Liss, Inc.