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Response of primary rabbit kidney proximal tubule cells to estrogens
Author(s) -
Han Ho Jae,
Jung Jee Chang,
Taub Mary
Publication year - 1999
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199901)178:1<35::aid-jcp5>3.0.co;2-p
Subject(s) - medicine , endocrinology , estrogen , biology , tamoxifen , estrogen receptor , testosterone (patch) , alkaline phosphatase , cell growth , steroid , receptor , cell culture , hormone , chemistry , enzyme , biochemistry , cancer , genetics , breast cancer
The effects of estrogens on the growth and function of primary rabbit kidney proximal tubule (RPT) cells have been examined in hormonally defined phenol red–free medium. 17β‐estradiol was observed to stimulate growth at dosages as low as 10 −10 M. The growth stimulatory effects of 17β‐estradiol were mitigated in the presence of hydrocortisone, suggesting that these two steroid hormones acted at least in part by common mechanisms. The effects of other steroids known to interact with the estrogen receptor were examined. Alpha estradiol was found to be growth stimulatory over a concentration range of 10 −9 to 10 −8 M, albeit to a lower extent than beta estradiol. In addition, the anti‐estrogen tamoxifen was also growth stimulatory (unlike the case with the human mammary tumor cell line MCF‐7). The effects of several metabolic precursors of 17β‐estradiol were examined, including testosterone, which was growth stimulatory, and progesterone, which was growth inhibitory. The growth stimulatory effects of 17β‐estradiol, alpha estradiol, and tamoxifen could possibly be explained by their interaction with an estrogen receptor. Indeed, metabolic labelling and immunoprecipitation studies indicated the presence of such an estrogen receptor in the primary cultures. The rate of biosynthesis of the estrogen receptor was found to be affected by the presence of exogenously added 17β‐estradiol. 17β‐estradiol was also observed to increase the activity of two brush border enzymes, alkaline phosphatase and gamma glutamyl transpeptidase, during the growth phase of the primary cultures. J Cell Physiol 178:35–43, 1999. © 1999 Wiley‐Liss, Inc.