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Cloning of murine CDK9/PITALRE and its tissue‐specific expression in development
Author(s) -
Bagella Luigi,
MacLachlan Timothy K.,
Buono Russell J.,
Pisano M. Michele,
Giordano Antonio,
De Luca Antonio
Publication year - 1998
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199811)177:2<206::aid-jcp2>3.0.co;2-r
Subject(s) - biology , kinase , cyclin dependent kinase 5 , c2c12 , microbiology and biotechnology , cellular differentiation , cyclin dependent kinase 1 , protein kinase a , p19 cell , cyclin dependent kinase 9 , complementary dna , myocyte , cell cycle , cyclin dependent kinase 2 , cell , biochemistry , gene , adult stem cell , myogenesis
The cdc2‐family of serine/threonine kinases and their binding partners recently were implicated in developmental roles. We previously cloned a cdc2‐related kinase, cdk9/PITALRE, that is able to phosphorylate the retinoblastoma protein in vitro. We describe here the cloning and the characterization of the mouse homolog of cdk9/PITALRE. The murine cDNA is 98% identical with humans and is expressed at high levels in brain and kidney tissues. The kinase activity and protein expression of cdk9/PITALRE were highest in terminally differentiated tissues such as the muscle and brain. In situ immunohistology and immunofluorescence detected cdk9/PITALRE protein not only within terminally differentiated cells such as muscle and neuronal cells, but also in proliferating cells. C2C12 and P19 cells induced to differentiate along muscle and neural lineages peaked in cdk9/PITALRE kinase activity at the end of differentiation. These results suggest that, among other roles, cdk9/PITALRE plays a role not unlike cdk5 in the differentiation of certain cell types. J. Cell. Physiol. 177:206–213, 1998. © 1998 Wiley‐Liss, Inc.