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Evidence for the participation of nerve growth factor and its low‐affinity receptor (p75 NTR ) in the regulation of the myogenic program
Author(s) -
Seidl Klaus,
Erck Christian,
Buchberger Astrid
Publication year - 1998
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199807)176:1<10::aid-jcp2>3.0.co;2-b
Subject(s) - c2c12 , myogenesis , biology , nerve growth factor , neurotrophin , myocyte , neurite , cellular differentiation , microbiology and biotechnology , receptor , autocrine signalling , trk receptor , in vitro , biochemistry , gene
We have studied expression and function of neurotrophins and their receptors during myogenic differentiation of C2C12 cells, a clonal cell line derived from mouse muscle that is capable of in vitro differentiation. The genes coding for nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF) and their common low‐affinity receptor p75 neurotrophin receptor (p75 NTR ) were shown to be expressed in C2C12 myoblasts and downregulated during myogenic differentiation and fusion into myotubes. Cocultures with dorsal root ganglia from day 8 chick embryos revealed neurite‐promoting activities of C2C12 cells that ceased with myogenic differentiation. These data suggest a temporal and developmental window for the effect of myogenic cell‐derived neurotrophins on neuronal as well as on myogenic cell populations. NGF was shown to increase DNA synthesis and cell growth of C2C12 myoblasts and to enhance myogenic differentiation in this cell line. We present evidence that NGF‐mediated processes take place at stages preceding myogenic differentiation. Enhanced muscle differentiation was also seen in p75 NTR ‐overexpressing C2C12 myoblasts which maintained high levels of receptors but ceased to produce NGF during differentiation. In contrast, when exogenous NGF was present at the onset of myogenic differentiation of receptor‐overexpressing cells, muscle cell development was strongly repressed. This indicates that downregulation of p75 NTR is necessary for guiding myogenic cells towards terminal differentiation. Since none of the trk high‐affinity neurotrophin receptors could be demonstrated in C2C12 cells, we conclude that NGF mediates its nonneurotrophic effect via its low‐affinity receptor in an autocrine fashion. J. Cell. Physiol. 176:10–21, 1998. © 1998 Wiley‐Liss, Inc.