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Differential modulation of hepatocyte growth factor‐stimulated motility by transforming growth factor β1 on rat liver epithelial cells in vitro
Author(s) -
Stolz Donna Beer,
Michalopoulos George K.
Publication year - 1998
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199804)175:1<30::aid-jcp4>3.0.co;2-d
Subject(s) - hepatocyte growth factor , motility , transforming growth factor , microbiology and biotechnology , in vitro , hepatocyte , growth factor , chemistry , modulation (music) , biology , biochemistry , receptor , physics , acoustics
We have previously shown that transforming growth factor‐β1 (TGF‐β1) enhances the epidermal growth factor‐ (EGF) and transforming growth factor‐α (TGF‐α)‐stimulated motility of rat hepatocytes in an extracellular matrix (ECM)‐dependent fashion (Stolz and Michalopoulos, 1997, J. Cell. Physiol., 170: 57–68). We have extended this study to examine the effects of TGF‐β1 on hepatocyte growth factor (HGF) and EGF‐stimulated motility of rat nonparenchymal liver epithelial cells (RLECs) in vitro and determined that chemotaxis, scattering, and monolayer wound healing by EGF was synergistically enhanced by TGF‐β1 on all ECMs examined. However, HGF‐based motility, unlike EGF‐stimulated motility, was modulated in an assay‐dependent manner by TGF‐β1. HGF‐stimulated chemotaxis was dramatically decreased by addition of TGF‐β1, but wound healing was synergistically enhanced by TGF‐β1 on all ECMs examined. HGF‐based scattering was not consistently affected by TGF‐β1 on any ECM tested except on laminin, where scattering was often reduced by the concomitant addition of TGF‐β1. TGF‐β1 enhanced the motility associated with monolayer wound healing by HGF or EGF independent of DNA synthesis, because tritiated thymidine uptake was consistently reduced by 60% in the presence of TGF‐β1. The data indicate that HGF and EGF motility do not follow redundant signal‐transduction pathways and that specific growth factor motility‐related events, as measured by wound healing, scattering, and chemotaxis, are modulated independently by ECM and TGF‐β1. J. Cell. Physiol. 175:30–40, 1998. © 1998 Wiley‐Liss, Inc.