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Properties of primary murine stroma induced by macrophage colony‐stimulating factor
Author(s) -
Yamada Muneo,
Suzu Shinya,
Akaiwa Eriko,
Wakimoto Noriko,
Hatake Kiyohiko,
Motoyoshi Kazuo,
Shimamura Seiichi
Publication year - 1997
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199710)173:1<1::aid-jcp1>3.0.co;2-r
Subject(s) - macrophage colony stimulating factor , biology , stromal cell , microbiology and biotechnology , clone (java method) , bone marrow , colony stimulating factor , stem cell factor , granulocyte macrophage colony stimulating factor , cell culture , growth factor , macrophage , cytokine , stem cell , haematopoiesis , immunology , receptor , in vitro , cancer research , biochemistry , genetics , dna
The ability of purified human macrophage colony‐stimulating factor (M‐CSF) to accelerate the formation of stromal cells from murine bone marrow cells was investigated. The liquid culture of the marrow cells with M‐CSF resulted in the formation of monolayers of macrophages on day 7. When the M‐CSF was removed on that day and the residual adherent cells were cultured in the absence of M‐CSF for an additional 7 days, many colonies appeared with cells that were morphologically distinguishable from M‐CSF‐derived macrophages. The appearance of the colonies was dependent on the concentration of M‐CSF used at the beginning of the culture. Each colony was isolated as a single clone and analyzed. All clones were negative for esterase staining. These cells did not express M‐CSF receptor mRNA and did not show a mitogenic response to M‐CSF. On the contrary, these cells could be stimulated to proliferate by fibroblast growth factor and platelet‐derived growth factor. The polymerase chain reaction analysis of these cells demonstrated constitutive expression of mRNA for M‐CSF, stem cell factor, and interleukin (IL)‐1, but not IL‐3. Some clones expressed mRNA for granulocyte/M‐CSF and IL‐6. We also examined the ability of the cells to maintain murine bone marrow high proliferative potential colony‐forming cells (HPP‐CFC) in a coculture system. Most of the clones showed a significant increase in total HPP‐CFC numbers after 2 weeks of coculture, although the extent of stimulation differed among clones. These results suggested that the colonies established by M‐CSF were composed of functional stromal cells that were phenotypically different from macrophages. J. Cell. Physiol. 173:1–9, 1997. © 1997 Wiley‐Liss, Inc.