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1,25‐dihydroxyvitamin D 3 selectively induces increased expression of the Na,K‐ATPase β1 subunit in avian myelomonocytic cells without a concomitant change in Na,K‐ATPase activity
Author(s) -
Billecocq Agnes,
Horne William C.,
Chakraborty Munmun,
Takeyasu Kunio,
Levenson Robert,
Baron Roland
Publication year - 1997
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199708)172:2<221::aid-jcp9>3.0.co;2-q
Subject(s) - protein subunit , atpase , concomitant , chemistry , v atpase , microbiology and biotechnology , enzyme , biology , biophysics , biochemistry , medicine , gene
Treatment of avian myelomonocytic cells with 1,25‐dihydroxyvitamin D 3 (1,25(OH)2D3) results in an approximately two fold increase in levels of Na,K‐ATPase β1 subunit mRNA and protein (both total and plasma membrane‐associated). The changes in β1 subunit expression occur in the absence of a detectable increase in expression of any of the three α subunit isoforms or in Na,K‐ATPase activity. The selective induction of the expression of the β subunit in avian myelomonocytic cells by 1,25(OH)2D3 reveals a previously unobserved feature of the regulation of Na,K‐ATPase expression, while the targeting of β subunit polypeptides to the plasma membrane in the absence of a corresponding increase in active Na,K‐ATPase suggests that, in these cells, transport of the β subunit to the plasma membrane may be independent of its binding to the α subunit. J. Cell. Physiol. 172:221–229, 1997. © 1997 Wiley‐Liss, Inc.