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Nitric oxide inhibits neutrophil β 2 integrin function by inhibiting membrane‐associated cyclic GMP synthesis
Author(s) -
Banick Paul D.,
Chen Qiping,
Xu Y. Anne,
Thom Stephen R.
Publication year - 1997
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199707)172:1<12::aid-jcp2>3.0.co;2-g
Subject(s) - nitric oxide , integrin , function (biology) , microbiology and biotechnology , cyclic gmp , chemistry , membrane , biochemistry , biology , cell , organic chemistry
The aim of this investigation was to identify the mechanism by which nitric oxide inhibits neutrophil β 2 integrin dependent adherence. Isolated rat neutrophils from blood and peritoneal exudates were exposed for 2 min to nitric oxide generated by diethylamine‐NO at rates between 1.6 and 138 nmol/min. Exposure to nitric oxide at rates less than 14 nmol/min had no effect on adherence. Exposure to 14 to 56 nmol nitric oxide/min inhibited β 2 integrin dependent adherence to endothelial cells, nylon columns, and fibrinogen‐coated plates, but higher concentrations had no significant effect on adherence. Adherence by β 2 integrins could be restored by incubating cells with dithioerythritol, phorbol 12‐myristate 13‐acetate, or 8‐bromo cyclic GMP. Elevations in cellular cyclic GMP concentration were associated with adherence, but this did not occur after cells were exposed to concentrations of nitric oxide that inhibited β 2 integrin‐dependent adherence. Elevations in cyclic GMP did occur after cells were incubated with dithioerythritol or phorbol 12‐myristate 13‐acetate. Concentrations of nitric oxide that inhibited β 2 integrin‐dependent adherence also inhibited catalytic activity of membrane associated guanylate cyclase and binding of atrial natriuretic peptide, but were insufficient to activate cytosolic guanylate cyclase. Nitric oxide did not inhibit neutrophil oxidative burst or degranulation, nor effect β 2 integrin expression or adherence that did not depend on β 2 integrins, nor cause oxidative stress identified in terms of cellular glutathione concentration or protein nitrotyrosine. The results indicate that nitric oxide inhibited β 2 integrins in a concentration‐dependent fashion by inhibiting cell‐surface transduction of signals linked to the activity of membrane‐bound guanylate cyclase. The inhibitory effect could be overcome by providing cells with cyclic GMP exogenously or by stimulating cytosolic guanylate cyclase. J. Cell. Physiol. 172:12–24, 1997. © 1997 Wiley‐Liss, Inc.