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Control of fibronectin receptor expression by fibronectin: Antisense fibronectin RNA downmodulates the induction of fibronectin receptor by transforming growth factor β1
Author(s) -
Rajagopal Sriram,
Huang Shuang,
Albitar Maher,
Chakrabarty Subhas
Publication year - 1997
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199702)170:2<138::aid-jcp5>3.0.co;2-p
Subject(s) - fibronectin , fndc5 , microbiology and biotechnology , extracellular matrix , receptor , biology , transforming growth factor , chemistry , biochemistry
The results of our previous studies of mouse embryo fibroblasts showed that fibronectin expression and fibronectin receptor expression are tightly coregulated and that fibronectin modulates expression of its receptor in response to treatment with the differentiation‐inducing agent N,N,‐dimethylformamide (Varani and Chakrabarty, 1990, J. Cell. Physiol., 143 :445–454; Huang et al., 1994, J. Cell. Physiol., 161 :470–482). We also found that transforming growth factor β1 (TGFβ1) induces a more differentiated phenotype in the epithelium‐derived human colon carcinoma cell line Moser and upregulates the expression of both fibronectin and its receptor (Huang and Chakrabarty, 1994, Int. J. Cancer, 57 :742–746). By expressing antisense fibronectin RNA in Moser cells, we have downregulated fibronectin mRNA expression and thus blocked the ability of TGFβ1 to induce fibronectin expression (Huang and Chakrabarty, 1994, J. Biol. Chem., 269 :28764–28768). In this study, we examined the effect of antisense fibronectin RNA expression on the induction of fibronectin receptor by TGFβ1 and tested the hypothesis that the induction of fibronectin expression by TGFβ1 is required for the induction of fibronectin receptor expression. Blocking fibronectin induction by TGFβ1 attenuated the ability of TGFβ1 to upregulate the expression of cell‐surface fibronectin receptors, α5β1 integrin expression, and adhesion to extracellular matrix fibronectin. We therefore conclude that induction of fibronectin expression is required for optimal upregulation of fibronectin receptor expression by TGFβ1. J. Cell. Physiol. 170:138–144, 1997. © 1997 Wiley‐Liss, Inc.