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Lactoferrin as a suppressor of cell migration of gastrointestinal cell lines
Author(s) -
Nakajima Mitsunari,
Shinoda Ichizo,
Samejima Yoshiko,
Miyauchi Hirofumi,
Fukuwatari Yasuo,
Hayasawa Hirotoshi
Publication year - 1997
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/(sici)1097-4652(199702)170:2<101::aid-jcp1>3.0.co;2-s
Subject(s) - lactoferrin , in vitro , chemistry , cell culture , cell , glycoprotein , cell migration , transferrin , caco 2 , biochemistry , hydrolysate , microbiology and biotechnology , biology , genetics , hydrolysis
The effects of lactoferrin (Lf), an iron‐binding glycoprotein, on cell migration were investigated. Lf inhibited the cell migration of three gastrointestinal cell lines (Caco‐2 cells, AGS cells, and IEC‐18 cells) in vitro. Both iron‐saturated (holo) and iron‐depleted (apo) Lf showed this inhibitory effect. Chelation of iron in the culture medium by desferrioxamine did not affect the activity of either form of Lf. A pepsin hydrolysate of Lf exhibited effectiveness similar to that of intact Lf. These results demonstrate a novel activity of Lf and suggest a potential role for this molecule in gastrointestinal wound healing, which is independent of its iron‐binding capacity. J. Cell. Physiol. 170:101–105, 1997. © 1997 Wiley‐Liss, Inc.